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The reduction in lamin A/C proteins was more than 90% complete as quantified by western blotting (Fig. 4j, k). We note that lamin A/C ‘knock out’ mice are viable for a few weeks after birth23 and that the lamin A/C knock down in cultured cells was not expected to cause…
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At the time I had a very stressful job, travelling four days each week, not exercising, and eating and drinking as much as I wanted. One night after a long week on the road I came home and, for the first time, saw what everyone else had seen. I was…